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| De Verdal, Hugues; O'Connell, Catherine Maeve; Mekkawy, Wagdy; Vandeputte, Marc; Chatain, Beatrice; Bégout, Marie-laure; Benzie, John A.h.. |
| Given the strong effects of behavioural hierarchies on growth in many cultured species and the key role of feed efficiency in aquaculture economics, understanding the nature of the interaction of these variables is important for the sustainability of aquaculture. The relationship between agonistic behaviour, growth and feed efficiency in Nile tilapia, Oreochromis niloticus, was studied by rearing 120 fish in eight aquaria. Fish were video-recorded to estimate the occurrence of agonistic behaviour during a fasting and a refeeding period. Growth, feed intake and feed conversion efficiency (FCE - calculated as the inverse of feed conversion ratio (FCR)) were subsequently measured individually for each fish. Fish showed 58% less agonistic traits during the... |
| Tipo: Text |
Palavras-chave: Agonstic behaviour; Feed efficiency; Nile tilapia; Performances; Correlation. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00483/59495/62363.pdf |
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| Blanc, Mélanie; Alfonso, Sebastien; Bégout, Marie-laure; Barrachina, Célia; Hyötyläinen, Tuulia; Keiter, Steffen H.; Cousin, Xavier. |
| Polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) are persistent organic pollutants still present in aquatic environments despite their total or partial ban. Previously, we observed that an environmentally realistic mixture of these compounds affects energy balance, growth, and reproduction in exposed zebrafish (F0), and behavior in their unexposed offspring (F1-F4). In the present work, we performed lipidomic and transcriptomic analyses on brains of zebrafish (F0-F2) from exposed and control lineages to identify molecular changes that could explain the observed phenotypes. The use of both technologies highlighted that F0 zebrafish displayed impaired mitochondrial function and lipid metabolism regulation (depletion in... |
| Tipo: Text |
Palavras-chave: Danio rerio; Persistent-organic pollutant; Neurotoxicity; Brain metabolism; RNA-Seq. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00646/75797/76720.pdf |
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| Cormier, Bettie; Gambardella, Chiara; Tato, Tania; Perdriat, Quentin; Costa, Elisa; Veclin, Cloé; Le Bihanic, Florane; Grassl, Bruno; Dubocq, Florian; Kärrman, Anna; Van Arkel, Kim; Lemoine, Soazig; Lagarde, Fabienne; Morin, Benedicte; Garaventa, Francesca; Faimali, Marco; Cousin, Xavier; Bégout, Marie-laure; Beiras, Ricardo; Cachot, Jerome. |
| Microplastics are ubiquitous in aquatic ecosystems, but little information is currently available on the dangers and risks to living organisms. In order to assess the ecotoxicity of environmental microplastics (MPs), samples were collected from the beaches of two islands in the Guadeloupe archipelago, Petit-Bourg (PB) located on the main island of Guadeloupe and Marie-Galante (MG) on the second island of the archipelago. These samples have a similar polymer composition with mainly polyethylene (PE) and polypropylene (PP). However, these two samples are very dissimilar with regard to their contamination profile and their toxicity. MPs from MG contain more lead, cadmium and organochlorine compounds while those from PB have higher levels of copper, zinc and... |
| Tipo: Text |
Palavras-chave: Environmental microplastics; Leachates; Early life stages; Aquatic organisms; Toxicity. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00661/77276/78711.pdf |
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| Alfonso, Sebastien; Sadoul, Bastien; Gesto, Manuel; Joassard, Lucette; Chatain, Beatrice; Geffroy, Benjamin; Bégout, Marie-laure. |
| Coping styles consist of a coherent set of individual physiological and behavioral differences in stress responses that are consistent across time and context. Such consistent inter-individual differences in behavior have already been shown in European sea bass (Dicentrarchus labrax), but the associated mechanisms are still poorly understood. Here, we combine physiological measurements with individual behavioral responses in order to characterize coping styles in fish. Fish were tagged and placed in a tank for group risk-taking tests (GRT) at 8 months of age to evaluate boldness using the proxy latency of leaving a sheltered area towards an open area. A subsample of these fish were individually challenged 16 months later using an open field test (OFT), in... |
| Tipo: Text |
Palavras-chave: Personality; HPI axis; Gene transcription; Neurotransmitters. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00491/60282/63708.pdf |
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| Vandeputte, Marc; Clota, Frederic; Sadoul, Bastien; Blanc, Marie-odile; Blondeau-bidet, Eva; Bégout, Marie-laure; Cousin, Xavier; Geffroy, Benjamin. |
| Temperature‐dependent sex determination (TSD) can be observed in multiple reptile and fish species. It is adaptive when varying environmental conditions advantage either males or females. A good knowledge of the thermosensitive period is key to understand how environmental changes may lead to changes in population sex ratio. Here, by manipulating temperature during development, we confirm that cold temperature (16°C) increases the proportion of fish that develop as females in European sea bass (Dicentrarchus labrax) until 56 days posthatching, but show that it has an opposite effect at later stages, with the proportion of males reaching ~90% after 230 days at 16°C. This is the first observation of opposite effects of temperature at different time periods... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; Fish; Sex ratio; Temperature-dependent sex determination; Thermosensitive period; TSD. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00658/76965/78184.pdf |
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| Le Bihanic, Florane; Clérandeau, Christelle; Cormier, Bettie; Crebassa, Jean-claude; Keiter, Steffen H.; Beiras, Ricardo; Morin, Bénédicte; Bégout, Marie-laure; Cousin, Xavier; Cachot, Jerome. |
| The role of polyethylene microplastics 4–6 μm size (MPs) in the toxicity of environmental compounds to fish early life stages (ELS) was investigated. Marine medaka Oryzias melastigma embryos and larvae were exposed to suspended MPs spiked with three model contaminants: benzo(a)pyrene (MP-BaP), perfluorooctanesulfonic acid (MP-PFOS) and benzophenone-3 (MP-BP3) for 12 days. There was no evidence of MPs ingestion but MPs agglomerated on the surface of the chorion. Fish ELS exposed to virgin MPs did not show toxic effects. Exposure to MP-PFOS decreased embryonic survival and prevented hatching. Larvae exposed to MP-BaP or MP-BP3 exhibited reduced growth, increased developmental anomalies and abnormal behavior. Compared to equivalent waterborne concentrations,... |
| Tipo: Text |
Palavras-chave: Marine medaka; Microplastics; Developmental toxicity; Benzo(a)pyrene; Perfluorooctanesulfonic acid; Benzophenone-3. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00619/73112/74844.pdf |
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| Ferrari, Sebastien; Rey, Sonia; Høglund, Erik; Øverli, Øyvind; Chatain, Beatrice; Mackenzie, Simon; Bégout, Marie-laure. |
| Individual stress coping style (reactive, intermediate and proactive) was determined in 3 groups of 120 pit tagged European seabass using the hypoxia avoidance test. The same three groups (no change in social composition) were then reared according to the standards recommended for this species. Then, 127 days later, individuals initially characterized as reactive, intermediate or proactive were submitted to an acute confinement stress for 30 min. Blood samples were taken to measure plasma cortisol levels 30 min (Stress30) or 150 min (Stress150) after the end of the confinement stress. Individuals were then sacrificed to sample the telencephalon in order to measure the main monoamines and their catabolites (at Stress30 only). Individuals from Stress150 were... |
| Tipo: Text |
Palavras-chave: Fish; Behavior; Physiology; Personality; Brain; Transcriptomic. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00601/71330/69760.pdf |
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| Alfonso, Sebastien; Sadoul, Bastien; Cousin, Xavier; Bégout, Marie-laure. |
| In aquaculture, fish are exposed to unavoidable stressors that can be detrimental for their health and welfare. However, welfare in farmed fish can be difficult to assess, and, so far, no standardized test has been universally accepted as a welfare indicator. This work contributes to the establishment of behavioural welfare indicators in a marine teleost in response to different water quality acute stressors. Groups of ten fish were exposed to high Total Ammonia Nitrogen concentration (High TAN, 18 mg.L-1), Hyperoxia (200 % O2 saturation), Hypoxia (20 % O2 saturation), or control water quality (100% O2 saturation and TAN < 2.5 mg.L-1) over 1 hour. Fish were then transferred in a novel environment for a group behaviour test under the same water quality... |
| Tipo: Text |
Palavras-chave: Fish; Welfare; Water quality; Behaviour; Stress. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00612/72399/71344.pdf |
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| Alfonso, Sebastien; Peyrafort, Manon; Cousin, Xavier; Bégout, Marie-laure. |
| Coping style is defined as a set of individual physiological and behavioural characteristics consistent across time and context. In the zebrafish Danio rerio, as well as in many other animals, several covariations have been established between behavioural, physiological, and molecular responses. However, not many studies have addressed the consistency of behavioural responses over time starting at the larval stage. Therefore, we aimed to improve our understanding of behavioural consistency across context and over time in zebrafish from the larval to juvenile stages. We conducted two distinct experiments: a larval stage experiment (from 8 to 21 days post fertilization, dpf) and a juvenile stage experiment (from 21 to 60 dpf). On one hand, the larval... |
| Tipo: Text |
Palavras-chave: Coping style; Fish; Personality; Vital staining. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00614/72598/71598.pdf |
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